Table 1 Primers used in this study
From: Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans
Name | Sequence (5′ to 3′) | Truncation sites on Chr4 |
|---|---|---|
For truncation constructs | ||
 KC32 | TCG CTC GAG ACT TGG GAT AAG GAG AGC AAA | 969.925 (1) |
 KC33 | TCG GGT ACC TGT TGC TGA CGA TGT TGA | |
 KC34 | TCG GGT ACC ATT TGT ACT GTT TTG CGT CTG | 1002.852 (2) |
 KC35 | TCG CTC GAG GAC TCA CCA ATA GTT CAA GGC A | |
 KC432 | GGA TCC CTG CTA AAC GAT ACC AGC AAT TAA CT | 1102.087 (3) |
 KC433 | GAG CTC TGT GTC CAT CAA AGC CCA AT | |
 KC434 | GGA TCC GCA CAA CCA ATT GAA GCT GGT A | 1201.989 (4) |
 KC435 | GAG CTC CGT TTC TAA CAG TTC TTG CAC G | |
 KC436 | GGA TCC CAG ACG ATA AGT GAA TAT CTC G | 1301.783 (5) |
 KC437 | GAG CTC GGA ATA CTA TGT GTG TCA AGG GGA | |
 KC136 | TCG GGT ACC AAT CAC CAT CCG ACG AGT ACT T | 1369.883 (6) |
 KC137 | TCG CTC GAG TCA TGT GCT TTT GTC TC | |
 KC139 | TCG GGT ACC TAG AAT GGC CAT GTT GCA TAG TC | 1529.969 (7) |
 KC140 | TCG CTC GAG CAC ATC GTA CCG TAA TTG AAA | |
 KC172 | GGT ACC GAT CTT GCT TTG TTC CTA CAA A | 1542.907 (8) |
 KC173 | CTC GAG CCA AAG CAA CAG CCG AAT ACT A | |
For verification of truncation junctions | ||
 KC49 | CCA GCA AGA CTT TGC AAT GT | 969.925 |
 KC48 | GAT TTA AAG CTC CAT GTG CCA | 1002.852 |
 KC442 | CTG GCA ATG AAC GAT CAA GT | 1102.087 |
 KC443 | ACC AGC ATG ATA GAA CCC AA | 1201.989 |
 KC444 | CAA GAT AGC TAG AGC TCA GA | |
 KC445 | AGT CCT ACG TAG GTC AGT AA | 1301.783 |
 KC138 | TCC AAG AAA CAA TTT GAA AAT CCA | 1369.883 |
 KC141 | CCA CAA ATC AAT TGC AAA C | 1529.969 |
 KC174 | TTC GTT CGA TGC TCG TAT TCT AG | 1542.907 |
 KC27 | TTG ATG CAT TAA ACA CCT TGA | URA3 flipper |
 KC102 | TCC TAT TCC TTC TCC TTA TGG C | |