Skip to main content

Table 1 Primers used in this study

From: Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Name Sequence (5′ to 3′) Truncation sites on Chr4
For truncation constructs
 KC32 TCG CTC GAG ACT TGG GAT AAG GAG AGC AAA 969.925 (1)
 KC33 TCG GGT ACC TGT TGC TGA CGA TGT TGA
 KC34 TCG GGT ACC ATT TGT ACT GTT TTG CGT CTG 1002.852 (2)
 KC35 TCG CTC GAG GAC TCA CCA ATA GTT CAA GGC A
 KC432 GGA TCC CTG CTA AAC GAT ACC AGC AAT TAA CT 1102.087 (3)
 KC433 GAG CTC TGT GTC CAT CAA AGC CCA AT
 KC434 GGA TCC GCA CAA CCA ATT GAA GCT GGT A 1201.989 (4)
 KC435 GAG CTC CGT TTC TAA CAG TTC TTG CAC G
 KC436 GGA TCC CAG ACG ATA AGT GAA TAT CTC G 1301.783 (5)
 KC437 GAG CTC GGA ATA CTA TGT GTG TCA AGG GGA
 KC136 TCG GGT ACC AAT CAC CAT CCG ACG AGT ACT T 1369.883 (6)
 KC137 TCG CTC GAG TCA TGT GCT TTT GTC TC
 KC139 TCG GGT ACC TAG AAT GGC CAT GTT GCA TAG TC 1529.969 (7)
 KC140 TCG CTC GAG CAC ATC GTA CCG TAA TTG AAA
 KC172 GGT ACC GAT CTT GCT TTG TTC CTA CAA A 1542.907 (8)
 KC173 CTC GAG CCA AAG CAA CAG CCG AAT ACT A
For verification of truncation junctions
 KC49 CCA GCA AGA CTT TGC AAT GT 969.925
 KC48 GAT TTA AAG CTC CAT GTG CCA 1002.852
 KC442 CTG GCA ATG AAC GAT CAA GT 1102.087
 KC443 ACC AGC ATG ATA GAA CCC AA 1201.989
 KC444 CAA GAT AGC TAG AGC TCA GA
 KC445 AGT CCT ACG TAG GTC AGT AA 1301.783
 KC138 TCC AAG AAA CAA TTT GAA AAT CCA 1369.883
 KC141 CCA CAA ATC AAT TGC AAA C 1529.969
 KC174 TTC GTT CGA TGC TCG TAT TCT AG 1542.907
 KC27 TTG ATG CAT TAA ACA CCT TGA URA3 flipper
 KC102 TCC TAT TCC TTC TCC TTA TGG C