Fig. 5
From: Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans
Verification of truncation 5 on chromosome 4. A Karyotypic verification using CHEF. Sc, S. cerevisiae chromosomes size marker, three relevant chromosome sizes are indicated by arrows at 1532 kb, 1091 kb, and 924 kb respectively; P, wild type strain CAF4-2; Trn.5, chromosomes of the strain carrying truncation 5 at 1301.783 kb position on Chr4. The size of the truncated version of Chr4 (~ 1305 kb) is approximately 300 kb shorter than intact Chr4 and runs above Chr5 (Chr5 size---1191 kb). B PCR verification of truncation 5 with specific primers KC445/KC27. Lane M, 1 kb DNA ladder; lane P, PCR for wild type strain (no PCR product amplified); lane Trn.5, PCR for truncation junction producing 1.5 kb PCR product (expected size)