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Table 1 Effect of Agrobacterium co-cultivation of callus at different ages after its initiation on survival frequency at different stages of in vitro culture in two maize genotypes

From: Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

Age of callus Genotype Subculture Selection-I Selection-II Regeneration
0 days NM74C 42.00 ± 7.00 4.66 ± 1.52 1.00 ± 1.00 0.00 ± 0.00
NM5884 52.66 ± 3.51 15.66 ± 2.08 9.66 ± 2.08 4.33 ± 0.57
10 days NM74C 48.5 ± 5.77 9.28 ± 4.04 1.42 ± 0.57 0.00 ± 0.00
NM5884 67.00 ± 4.93 33.00 ± 5.29 16.75 ± 2.51 7.50 ± 1.73
20 days NM74C 66.0 ± 6.35 30.17 ± 2.88 4.31 ± 2.88 1.20 ± 0.50
NM5884 82.63 ± 2.08 67.63 ± 3.04 45.69 ± 8.02 25.13 ± 6.80
30 days NM74C 54.33 ± 6.50 12.66 ± 2.30 2.50 ± 1.00 1.06 ± 0.00
NM5884 81.00 ± 5.29 57.17 ± 6.02 38.66 ± 4.72 18.83 ± 1.52
40 days NM74C 46.8 ± 5.85 9.83 ± 2.08 2.16 ± 0.57 0.00 ± 0.00
NM5884 75.33 ± 7.37 43.33 ± 7.63 26.50 ± 5.29 9.66 ± 1.52