Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

Journal of Genetic Engineering and Biotechnology

Table 1 Effect of Agrobacterium co-cultivation of callus at different ages after its initiation on survival frequency at different stages of in vitro culture in two maize genotypes

Age of callus	Genotype	Subculture	Selection-I	Selection-II	Regeneration
0 days	NM74C	42.00 ± 7.00	4.66 ± 1.52	1.00 ± 1.00	0.00 ± 0.00
0 days	NM5884	52.66 ± 3.51	15.66 ± 2.08	9.66 ± 2.08	4.33 ± 0.57
10 days	NM74C	48.5 ± 5.77	9.28 ± 4.04	1.42 ± 0.57	0.00 ± 0.00
10 days	NM5884	67.00 ± 4.93	33.00 ± 5.29	16.75 ± 2.51	7.50 ± 1.73
20 days	NM74C	66.0 ± 6.35	30.17 ± 2.88	4.31 ± 2.88	1.20 ± 0.50
20 days	NM5884	82.63 ± 2.08	67.63 ± 3.04	45.69 ± 8.02	25.13 ± 6.80
30 days	NM74C	54.33 ± 6.50	12.66 ± 2.30	2.50 ± 1.00	1.06 ± 0.00
30 days	NM5884	81.00 ± 5.29	57.17 ± 6.02	38.66 ± 4.72	18.83 ± 1.52
40 days	NM74C	46.8 ± 5.85	9.83 ± 2.08	2.16 ± 0.57	0.00 ± 0.00
40 days	NM5884	75.33 ± 7.37	43.33 ± 7.63	26.50 ± 5.29	9.66 ± 1.52