|Biomaterial||Cell type||Transfection system||Change in transfection efficiency||Ref.|
|Coverslips coated with a lipid film, having 3D-bicontinuous cubic, 2D-inverted hexagonal, or 1D-lamellar nanostructures||HeLa-Luc cell line, stably expressing the luciferase||siRNA directly loaded into the lipid film||
Luciferase activity (luminescence/mg protein) was 4 × 105 in the untreated control.|
Highest gene silencing efficacy was for 3D-bicontinuous phase (luciferase activity ∼ 1.5. × 105).
In 2D-hexagonal and 1D-lamellar phases, luciferase activity was almost 2.5 × 105.
Bolus delivery (Lipofectamine™) showed gene silencing efficacy similar to that of 3D-bicontinuous phase.
|Micropatterns with different diameter and aspect ratio||Human MSCs||Cationic polyplexes||
Highest transfection efficiency was 20%, for ellipses with aspect ratio 8:1 and surface area 80 μm.|
Lowest transfection efficiency was 2%, for circular micropattern with a diameter of 20 μm.
|Nanogrooved and nanopillar surfaces with different width/diameter and height||Normal human lung fibroblasts||Lipofectamine™ 2000||
Highest % of transfection was 50%, in grooves 500 nm in width and 150 nm in height and in nanopillars with a diameter of 500 nm and a width of 150 nm.|
In flat surfaces, % transfection was ∼ 30%
|Nanogrooved surfaces with different deep and depth||C2C12 skeletal myoblasts||jetPEI® (Polyplus, US)|| Decrease of ∼ 73% in transfection efficiency, on nanogrooved patterns of 400 nm groove width and 400 nm depth, compared to flat surfaces.|
Decrease of ∼ 90% on nanogrooved pattern of 800 nm groove width and 500 nm depth, compared to flat surfaces.
|Nanogratins and nanopillar surfaces||Human MSCs||Lipofectamine™ 2000||Highest % transfection was at 3.3%, for cells on grooves of 250 nm width. In flat surfaces, % transfection was 1.8%|||
|SiNWs||Jurkat, L1.2 and GPE86||Naked DNA|| % of transfection was ∼ 20%, 20% and 5% in GPE86, L1.2 and Jurkat respectively.|
% transfection was between 0% and 1%, in flat surfaces for all cell types.
|SiNWs with different heights||Human dental pulp stem cells||Naked DNA|| % transfection was ∼ 90% for SiNW with heights of 1.2 and 3.5 μm.|
% transfection was less than 10% for SiNW with heights of 0.4 μm and 6.3 μm.
|SiNWs||Mouse embryonic stem cells||Naked DNA||Transfection efficiency lower than 1%.|||
|Ethanolamine-functionalised SiNWs||HeLa||Naked DNA||Luciferase expression (luminescence/mg protein) was 106 in ethanolamine-functionalised siNWs, compared to 104 in non-functionalised siNWs|||
|Nanopillars of different diameter||C2C12 skeletal myoblasts||jetPRIME® or Lipofectamine™|| For jetPRIME®, luciferase expression (luminescence/mg protein) was ∼ 8 × 103 for pillars with a diameter of 1000 nm, whereas in flat surfaces it was 3 × 103|
For Lipofetamine™, luciferase expression was 1 × 103 in nanopillars with a diameter 1000 nm and 5 × 103 in flat surfaces.