Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Munasinghe, Sachithri; Somaratne, Seneviratnege; Weerakoon, Shyama; Ranasinghe, Chandani

doi:10.1186/s43141-021-00187-2

Journal of Genetic Engineering and Biotechnology

Table 4 Effect of biological elicitors on Gyrinops walla cell suspension

From: Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Type of elicitation	Concentration (mg/L)	Incubation period (hours)	Area percentage of
Type of elicitation	Concentration (mg/L)	Incubation period (hours)	γ-Selinene	β-Caryophyllene	α-Cardinol	α-Guaiene
Control	0	0	0.01 (0.005)^hi	0.01 (0.001)^h	0.01 (0.005)ⁱ	0.01 (0.005)^g
PP	6.0	0.5	0.01 (0.005)^hi	0.01 (0.001)^h	0.01 (0.005)ⁱ	0.01 (0.005)^g
PP	6.0	2	0.02 (0.005)^hi	0.01 (0.005)^h	0.08 (0.005)ⁱ	0.04 (0.001)^gf
PP	6.0	4	0.09 (0.005)^gf	0.07 (0.02)^h	0.83 (0.07)^d	0.04 (0.01)^gf
PP	6.0	6	0.15 (0.03)^ed	1.13 (0.07)^e	0.99 (0.01)^c	0.09 (0.01)^d
PP	6.0	12	0.19 (0.01)^d	1.45 (0.05)^c	0.81 (0.07)^de	0.15 (0.02)^cb
PP	6.0	24	0.09 (0.01)^gf	0.59 (0.05)^e	0.24 (0.02)^f	0.09 (0.01)^d
PP	6.0	72	0.01 (0.005)^hi	0.23 (0.06)^f	0.02 (0.002)ⁱ	0.02 (0.001)^g
PP	8.0	0.5	0.01 (0.005)ⁱ	0.01 (0.001)^h	0.01 (0.005)ⁱ	0.01 (0.005)^g
PP	8.0	2	0.06 (0.04)^hgi	0.01 (0.005)^h	0.08 (0.005)ⁱ	0.06 (0.02)^edf
PP	8.0	4	0.13 (0.03)^edf	0.07 (0.02)^h	0.85 (0.05)^d	0.07 (0.02)^ed
PP	8.0	6	0.25 (0.04)^c	1.05 (0.09)^b	0.99 (0.01)^c	0.16 (0.01)^b
PP	8.0	12	0.44 (0.02)^a	1.50 (0.02)^b	1.53 (0.05)^a	0.21 (0.03)^a
PP	8.0	24	0.14 (0.04)^edf	0.20 (0.005)^f	0.14 (0.01)^h	0.12 (0.02)^c
PP	8.0	72	0.02 (0.001)ⁱ	0.19 (0.02)^f	0.02 (0.01)ⁱ	0.02 (0.002)^g
PP	10.0	0.5	0.003 (0.001)ⁱ	0.01 (0.001)^h	0.01 (0.005)ⁱ	0.01 (0.002)^g
PP	10.0	2	0.08 (0.01)^hgf	0.01 (0.005)^h	0.13 (0.01)^h	0.06 (0.02)^edf
PP	10.0	4	0.17 (0.02)^d	0.07 (0.02)^h	0.93 (0.04)^c	0.07 (0.02)^ed
PP	10.0	6	0.24 (0.03)^c	1.75 (0.04)^a	0.99 (0.01)^c	0.16 (0.01)^b
PP	10.0	12	0.43 (0.01)^ab	1.45 (0.05)^d	1.54 (0.03)^a	0.21 (0.03)^a
PP	10.0	24	0.12 (0.02)^edf	0.93 (0.05)^d	0.14 (0.03)^h	0.12 (0.02)^c
PP	10.0	72	0.02 (0.002)ⁱ	0.20 (0.05)^f	0.02 (0.001)ⁱ	0.02 (0.004)^g
LT	8.0	0.5	0.01 (0.005)ⁱ	0.01 (0.003)^h	0.01 (0.005)ⁱ	0.01 (0.004)^g
LT	8.0	2	0.10 (0.01)^egf	0.01 (0.005)^h	0.43 (0.01)^f	0.04 (0.005)^ef
LT	8.0	4	0.14 (0.04)^edf	0.85 (0.03)^f	0.75 (0.02)^fh	0.07 (0.02)^ed
LT	8.0	6	0.37 (0.02)^b	0.99 (0.01)^d	0.92 (0.02)^c	0.15 (0.03)^cb
LT	8.0	12	0.22 (0.01)^c	1.05 (0.03)^e	1.26 (0.02)^b	0.15 (0.03)^cb
LT	8.0	24	0.11 (0.02)^edf	0.97 (0.02)^d	0.35 (0.02)^f	0.24 (0.04)^a
LT	8.0	72	0.08 (0.01)^hgf	0.45 (0.05)^de	0.01 (0.001)ⁱ	0.03 (0.005)^g
LT	10.0	0.5	0.01 (0.005)ⁱ	0.01 (0.001)^h	0.01 (0.004)ⁱ	0.01 (0.005)^g
LT	10.0	2	0.08 (0.005)^hgf	0.01 (0.005)^h	0.25 (0.02)^h	0.04 (0.001)^ef
LT	10.0	4	0.15 (0.02)^ed	0.75 (0.03)^e	0.84 (0.04)^cd	0.06 (0.01)^edf
LT	10.0	6	0.36 (0.02)^b	0.99 (0.01)^d	0.95 (0.03)^c	0.12 (0.02)^c
LT	10.0	12	0.22 (0.01)^c	0.85 (0.03)^gf	0.81 (0.02)^d	0.13 (0.01)^cb
LT	10.0	24	0.12 (0.02)^edf	0.27 (0.02)^fg	0.23 (0.02)^h	0.05 (0.003)^ed
LT	10.0	72	0.02 (0.002)ⁱ	0.05 (0.03)^h	0.01 (0.003)ⁱ	0.02 (0.005)^g

PP, Phaeocremonium parasitica; TV, Trichoderma viride; and LT, Lasidiplodia theobromae. The mean values are followed by standard deviation within parentheses. The same letter along the columns indicates no statistically significant difference at p ≤ 0.05

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