Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Munasinghe, Sachithri; Somaratne, Seneviratnege; Weerakoon, Shyama; Ranasinghe, Chandani

doi:10.1186/s43141-021-00187-2

Journal of Genetic Engineering and Biotechnology

Table 3 Effect of biological elicitors on Gyrinops walla calli

From: Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Type of elicitation	Concentration (mg/L)	Incubation period (weeks)	Area percentage of
Type of elicitation	Concentration (mg/L)	Incubation period (weeks)	γ-Selinene	β-Caryophyllene	α-Cardinol	α-Guaiene
Control	0	0	0.01 (0.005)ⁿ	0.01 (0.002)^z	0.01 (0.006)^xy	0.01 (0.004)^op
PP	25	2	0.01 (0.005)ⁿ	0.01 (0.002)^z	0.01 (0.006)^xy	0.01 (0.004)^op
PP	25	4	0.05 (0.002)^mn	0.07 (0.02)^z	0.23 (0.02)^wv	0.23 (0.02)ⁿ
PP	25	6	0.15 (0.05)^jk	0.25 (0.02)^xw	0.75 (0.01)^onp	0.70 (0.03)^ij
PP	25	8	0.32 (0.02)^gfh	0.87 (0.02)^jlimk	0.84 (0.02)^oqp	0.95 (0.03)^feg
PP	25	10	0.48 (0.01)^a	0.84 (0.04)^lonm	0.92 (0.02)^jlki	0.84 (0.04)^fhg
PP	25	12	0.23 (0.01)^ji	0.68 (0.02)^pq	0.67 (0.05)^rsqp	0.42 (0.01)^m
PP	25	14	0.08 (0.01)^mn	0.34 (0.02)^w	0.41 (0.005)^u	0.11 (0.02)^o
PP	50	2	0.01 (0.0005)ⁿ	0.06 (0.01)^z	0.06 (0.01)^xy	0.13 (0.02)^o
PP	50	4	0.10 (0.001)^l	0.66 (0.01)^rq	0.68 (0.02)^rsq	0.80 (0.03)^ih
PP	50	6	0.25 (0.04)^ji	1.04 (0.06)^fheg	0.90 (0.08)^jlkm	1.02 (0.06)^e
PP	50	8	0.35 (0.03)^efg	1.24 (0.06)^cd	1.00 (0.09)^ghi	1.49 (0.08)^c
PP	50	10	0.38 (0.03)^ef	1.01 (0.005)^fhg	0.96 (0.03)^jhi	1.17 (0.05)^d
PP	50	12	0.33 (0.01)^gfh	0.86 (0.01)^lnmk	0.77 (0.09)^onp	0.79 (0.05)^ij
PP	50	14	0.05 (0.004)^mn	0.40 (0.07)^w	0.44 (0.06)^ut	0.26 (0.07)ⁿ
PP	75	2	0.01 (0.005)ⁿ	0.08 (0.01)^z	0.43 (0.01)^ut	0.23 (0.01)ⁿ
PP	75	4	0.01 (0.005)ⁿ	0.96 (0.06)^jfhigk	0.94 (0.05)^jlki	0.85 (0.05)^fhg
PP	75	6	0.27 (0.02)^ih	1.25 (0.05)^c	1.28 (0.03)^ab	1.18 (0.07)^d
PP	75	8	0.46 (0.05)^bcd	1.53 (0.04)^a	1.53 (0.05)^c	1.48 (0.04)^a
PP	75	10	0.58 (0.02)^a	1.02 (0.06)^feg	1.55 (0.05)^a	1.09 (0.05)^b
PP	75	12	0.47 (0.04)^bc	0.89 (0.05)^ponm	1.06 (0.07)^gf	0.98 (0.10)^ef
PP	75	14	0.02 (0.005)ⁿ	0.53 (0.06)^st	0.72 (0.02)^rqp	0.52 (0.02)^ml
PP	100	2	0.01 (0.005)ⁿ	0.03 (0.005)^z	0.01 (0.005)^xy	0.02 (0.01)^op
PP	100	4	0.01 (0.005)ⁿ	0.56 (0.05)^sr	0.38 (0.03)^v	0.50 (0.10)^ml
PP	100	6	0.27 (0.02)^gih	1.15 (0.03)^ed	1.12 (0.03)^fe	0.71 (0.03)^ij
PP	100	8	0.35 (0.04)^efg	0.91 (0.03)^jfhig	1.37 (0.02)^b	0.97 (0.03)^ef
PP	100	10	0.47 (0.04)^bc	0.47 (0.05)^stu	1.15 (0.04)^de	0.78 (0.04)^ih
PP	100	12	0.35 (0.02)^ef	0.22 (0.01)^xw	0.99 (0.01)^jki	0.47 (0.04)^ml
PP	100	14	0.01 (0.005)^mn	0.08 (0.02)^z	0.47 (0.01)^ut	0.03 (0.002)^op
LT	75	2	0.01 (0.005)ⁿ	0.01 (0.001)^z	0.01 (0.004)^xy	0.03 (0.01)^op
LT	75	4	0.05 (0.01)^mn	0.23 (0.02)^wx	0.08 (0.01)^x	0.24 (0.03)ⁿ
LT	75	6	0.14 (0.04)^jk	0.76 (0.02)^jlmk	0.25 (0.02)^wv	0.70 (0.03)^ij
LT	75	8	0.32 (0.02)^gfh	0.84 (0.02)^ponq	0.84 (0.03)^jlkm	0.93 (0.04)^feg
LT	75	10	0.48 (0.01)^a	0.95 (0.03)^jlhigk	0.82 (0.02)^onm	0.84 (0.04)^fhg
LT	75	12	0.23 (0.01)^ji	0.67 (0.02)^rq	0.72 (0.02)^rsqp	0.45 (0.03)^m
LT	75	14	0.08 (0.005)^lm	0.41 (0.005)^w	0.35 (0.02)^v	0.15 (0.02)^o
LT	100	2	0.01 (0.005)ⁿ	0.37 (0.02)^z	0.62 (0.05)^s	0.03 (0.003)^op
LT	100	4	0.27 (0.02)^ih	1.06 (0.07)^ef	1.18 (0.03)^dce	0.71 (0.03)^ij
LT	100	6	0.41 (0.03)^bcde	1.31 (0.03)^ab	1.01 (0.07)^ghi	0.96 (0.05)^ef
LT	100	8	0.39 (0.06)d^ef	1.28 (0.06)^bc	0.58 (0.07)^t	0.86 (0.05)^fhg
LT	100	10	0.38 (0.03)^cde	0.97 (0.02)^jfhig	0.24 (0.03)^w	0.47 (0.02)^ml
LT	100	12	0.02 (0.01)ⁿ	0.17 (0.02)^lnmk	0.10 (0.01)^x	0.11 (0.01)^o
LT	100	14	0.006 (0.01)ⁿ	0.06 (0.02)^z	0.06 (0.01)^xy	0.05 (0.01)^op
TV	75	2	0.01 (0.005)ⁿ	0.06 (0.01)^z	0.06 (0.01)^xy	0.13 (0.02)^o
TV	75	4	0.09 (0.005)^l	0.68 (0.02)^prq	0.66 (0.01)^rs	0.80 (0.03)^ih
TV	75	6	0.23 (0.02)^ji	0.89 (0.06)^jlimk	1.04 (0.06)^gf	1.02 (0.06)^e
TV	75	8	0.35 (0.02)^efg	0.95 (0.04)^fhig	1.22 (0.02)^cd	1.47 (0.06)^c
TV	75	10	0.38 (0.02)^ef	0.96 (0.03)^jfhigk	1.01 (0.005)^ghi	1.17 (0.05)^d
TV	75	12	0.33 (0.01)^gfh	0.45 (0.02)^ponq	0.86 (0.01)^lnm	0.79 (0.05)^ih
TV	75	14	0.08 (0.01)^lmn	0.21 (0.01)^xw	0.44 (0.02)^ut	0.29 (0.05)ⁿ
TV	100	2	0.01 (0.005)ⁿ	0.01 (0.004)^z	0.01 (0.003)^xy	0.03 (0.01)^op
TV	100	4	0.05 (0.01)^mn	0.23 (0.02)^xw	0.06 (0.01)^xy	0.28 (0.06)ⁿ
TV	100	6	0.22 (0.02)^ji	0.76 (0.02)^jlmk	0.24 (0.04)^wv	0.70 (0.03)^ij
TV	100	8	0.32 (0.02)^gfh	0.84 (0.02)^ponq	0.92 (0.05)^jlkm	0.93 (0.05)^feg
TV	100	10	0.48 (0.01)^a	0.95 (0.02)^jlhik	0.86 (0.01)^nm	0.86 (0.05)^fhg
TV	100	12	0.23 (0.01)^ji	0.67 (0.02)^prq	0.68 (0.02)^rsq	0.43 (0.02)^m
TV	100	14	0.08 (0.01)^mn	0.41 (0.005)^w	0.34 (0.02)^t	0.15 (0.05)^o

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