Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Journal of Genetic Engineering and Biotechnology

Table 2 Effect of Chemical elicitors on Gyrinops walla cell suspension

Type of elicitation	Concentration	Incubation period (hours)	Area percentage of
Type of elicitation	Concentration	Incubation period (hours)	γ-Selinene	β-Caryophyllene	α-Cardinol	α-Guaiene
Control	0	0	0.01 (0.005)^e	0.01 (0.01)^e	0.01 (0.005)^f	0.01 (0.005)^d
Salicylic acid	0.5 μM	0.5	0.01 (0.005)^e	0.01 (0.01)^e	0.01 (0.005)^f	0.01 (0.005)^d
Salicylic acid	0.5 μM	2	0.08 (0.005)^de	0.01 (0.005)^e	0.13 (0.01)^f	0.06 (0.02)^c
Salicylic acid	0.5 μM	4	0.13 (0.03)^cd	0.05 (0.01)^e	0.94 (0.05)^cd	0.07 (0.02)^c
Salicylic acid	0.5 μM	6	0.28 (0.03)^b	2.07 (0.10)^b	1.02 (0.06)^c	0.16 (0.01)^b
Salicylic acid	0.5 μM	12	0.44 (0.02)^a	1.97 (0.02)^a	1.53 (0.05)^a	0.21 (0.03)^a
Salicylic acid	0.5 μM	24	0.14 (0.04)^c	0.41 (0.07)^cd	0.14 (0.03)^f	0.12 (0.02)^bc
Salicylic acid	0.5 μM	72	0.05 (0.04)^d	0.20 (0.05)^cd	0.02 (0.002)^f	0.02 (0.002)^d
Methyl jasmonate	0.1 mM	0.5	0.01 (0.005)^e	0.01 (0.001)^e	0.01 (0.005)^f	0.01 (0.005)^d
Methyl jasmonate	0.1 mM	2	0.09 (0.001)^cd	0.01 (0.005)^e	0.35 (0.10)^de	0.07 (0.002)^c
Methyl jasmonate	0.1 mM	4	0.18 (0.02)^c	0.90 (0.07)^d	0.71 (0.04)^e	0.04 (0.005)^cd
Methyl jasmonate	0.1 mM	6	0.37 (0.02)^a	2.07 (0.08)^b	0.91 (0.08)^cd	0.15 (0.03)^b
Methyl jasmonate	0.1 mM	12	0.25 (0.04)^b	1.07 (0.04)^c	1.26 (0.02)^b	0.15 (0.03)^b
Methyl jasmonate	0.1 mM	24	0.09 (0.005)^cd	0.99 (0.30)^d	0.86 (0.01)^e	0.25 (0.02)^a
Methyl jasmonate	0.1 mM	72	0.08 (0.004)^de	0.04 (0.01)^e	0.01 (0.003)^f	0.04 (0.01)^d

The mean values are followed by standard deviation within parentheses. The same letter along the columns indicates no statistically significant difference at p ≤ 0.05