Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Munasinghe, Sachithri; Somaratne, Seneviratnege; Weerakoon, Shyama; Ranasinghe, Chandani

doi:10.1186/s43141-021-00187-2

Journal of Genetic Engineering and Biotechnology

Table 1 Effect of Chemical elicitors on Gyrinops walla calli

From: Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation

Type of elicitation	Concentration	Incubation period (weeks)	Area percentage of
Type of elicitation	Concentration	Incubation period (weeks)	γ-Selinene	β-Aryophyllene	α-Cardinol	α-Guaiene
Control	0	0	0.00 (0.005)^g	0.13 (0.01)ⁱ	0.01 (0.005)^g	0.01 (0.005)^f
Salicylic acid	10 μM	2	0.00 (0.005)^g	0.13 (0.01)ⁱ	0.01 (0.005)^g	0.01 (0.005)^f
Salicylic acid	10 μM	4	0.10 (0.01)^ef	0.81 (0.05)^gh	0.86 (0.05)^e	0.07 (0.02)^d
Salicylic acid	10 μM	6	0.25 (0.04)^d	1.09 (0.04)^g	0.94 (0.05)^de	0.07 (0.02)^d
Salicylic acid	10 μM	8	0.46 (0.05)^b	2.67 (0.08)^b	1.53 (0.05)^b	0.16 (0.01)^c
Salicylic acid	10 μM	10	0.58 (0.02)^a	3.52 (0.06)^a	1.86 (0.06)^a	0.28 (0.02)^a
Salicylic acid	10 μM	12	0.47 (0.04)^b	2.41 (0.09)^c	1.55 (0.05)^b	0.21(0.03)^b
Salicylic acid	10 μM	14	0.07 (0.02)^fg	0.89 (0.09)^gh	0.01 (0.01)^g	0.01 (0.005)^f
Salicylic acid	100μM	2	0.01 (0.005)^g	0.01 (0.001)ⁱ	0.01 (0.003)^g	0.01 (0.005)^f
Salicylic acid	100 μM	4	0.01 (0.005)^g	0.01 (0.005)ⁱ	0.01 (0.005)^g	0.01 (0.003)^f
Salicylic acid	100 μM	6	0.27 (0.02)^d	1.33 (0.10)^f	0.87 (0.07)^e	0.05 (0.01)^de
Salicylic acid	100 μM	8	0.46 (0.05)^b	2.88 (0.09)^b	1.53 (0.05)^b	0.16 (0.01)^c
Salicylic acid	100 μM	10	0.58 (0.02)^a	3.58 (0.03)^a	1.86 (0.06)^a	0.28 (0.02)^a
Salicylic acid	100 μM	12	0.47 (0.04)^b	0.24 (0.09)^c	1.55 (0.05)^b	0.21 (0.03)^b
Salicylic acid	100 μM	14	0.01 (0.001)^g	0.01 (0.001)ⁱ	0.01 (0.001)^g	0.01 (0.005)^f
Methyl jasmonate	1 mM	2	0.01 (0.005)^g	0.01 (0.001)ⁱ	0.01 (0.005)^g	0.01 (0.005)^f
Methyl jasmonate	1 mM	4	0.10 (0.01)^ef	0.01 (0.005)ⁱ	0.86 (0.05)^e	0.07 (0.02)^d
Methyl jasmonate	1 mM	6	0.25 (0.04)^d	1.07 (0.04)^g	1.26 (0.02)^c	0.09 (0.01)^d
Methyl jasmonate	1 mM	8	0.41 (0.03)^bc	1.82 (0.05)^e	0.94 (0.05)^de	0.07 (0.01)^d
Methyl jasmonate	1 mM	10	0.40 (0.06)^c	2.08 (0.07)^d	0.91 (0.09)^d	0.15 (0.03)^c
Methyl jasmonate	1 mM	12	0.12 (0.02)^e	0.91 (0.06)^gh	0.32 (0.06)^f	0.15 (0.03)^c
Methyl jasmonate	1 mM	14	0.08 (0.01)^fg	0.43 (0.06)^h	0.01 (0.001)^g	0.02 (0.02)^ef
Methyl jasmonate	10mM	2	0.01 (0.005)^g	0.01 (0.01)ⁱ	0.01 (0.005)^g	0.01 (0.005)^f
Methyl jasmonate	10 mM	4	0.01 (0.005)^g	0.01 (0.005)ⁱ	0.01 (0.003)^g	0.01 (0.004)^f
Methyl jasmonate	10 mM	6	0.27 (0.02)^b	1.34 (0.12)^f	0.87 (0.07)^e	0.05 (0.04)^de
Methyl jasmonate	10 mM	8	0.46 (0.05)^b	3.59 (0.04)^a	1.86 (0.06)^a	0.16 (0.01)^c
Methyl jasmonate	10 mM	10	0.50 (0.02)^a	2.86 (0.13)^b	1.53 (0.05)^b	0.28 (0.02)^a
Methyl jasmonate	10 mM	12	0.47 (0.04)^b	2.41 (0.09)^c	1.55 (0.05)^b	0.21 (0.03)^b
Methyl jasmonate	10 mM	14	0.01 (0.01)^g	0.01 (0.01)ⁱ	0.01 (0.01)^g	0.01 (0.005)^f

The mean values are followed by standard deviation within parentheses. The same letter along the columns indicates no statistically significant difference at p ≤ 0.0

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