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Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Multiplex CRISPR/Cas9-mediated genome editing of the FAD2 gene in rice: a model genome editing system for oil palm

Fig. 2

A Schematic of OsFAD2-1 gene loci. Predicted secondary structures for (B) sgRNA1 and (C) sgRNA2. PCR amplification and validation of sgRNAs via in vitro Cas9 cleavage assay; D PCR product from amplification of sgRNAs template at ~ 130 bp (lane 1: sgRNA1, lane 2: sgRNA2) for in vitro transcription, E PCR amplification of rice target DNA region (OsFAD2T1T2) at ~ 917 bp, and (F) analysis of cleavage products for sgRNA1 at 312 bp and 582 bp (lane 1) and sgRNA2 at 614 bp and 280 bp (lane 2). Band size at ~ 130 bp in (F) at lanes 1 and 2 are the residue of sgRNA template. Red arrows indicate the band size at the expected molecular weight. Lane M is Trans2K® Plus II DNA Marker (TransGen Biotech, China)

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