Table 1 The sequences of primers T-ARMS-PCR based amplification of GDF9 gene mutation points

G1

IF(A)

334 CTGCAGCCAGATGACAGAGCTTTTCA 359

Mut

205

IR(G)

388 CGTATGCCTTATAGAGCCTCTTCATGTCGC 359

Wt

247

OF

168 GCCTGGCTCTGTTTTCCTATTAGCCTTG 188

Co

396

OR

537 TCTTCTTCCCTCCACCCATTAACCAATC 510

G4

IF(G)

296 TTCACATGTCTGTAAATTTTACATGTGAGG 325

Mut

212

IR(A)

350 GCTGAAGGATGCTGCAGCTGGTCGTT 325

Wt

261

OF

90 CAACAACTCCATTTCTTTTCCCTTTCCTG 118

Co

417

OR

506 TAGGCAGATAGCCCTCTC TTCTGGTCAG 479

G6

IF(A)

573 CAGCTCTGAATTGAAGAAGCCTCGGA 598

Mut

193

IR(G)

625 ATTCACTCAGATTGACTGAAGCTGGCAC 598

Wt

223

OF

403 TATCTGAACGACACAAGTGCTCAGGCTT 430

Co

362

OR

764 CTGGGACAGTCCCCTTTACAGTATCGAG 737

G7

IF(A)

688 AGTCAGCTGAAGTGGGACAACTGGAGTG 715

Mut

187

IR(G)

742 ATCGAGGGTTGTATTTGTGTGGGGCAAT 715

Wt

212

OF

556 AGAGACCAGGAGAGTGCCAGCTCTGAAT 583

Co

343

OR

898 CGATGGCCAAAACACTCAAAGGGCTATA 871

G8

IF(T)

763 AGGGCGGTCGGACATCGGTATGGATT 788

Mut

146

IR(C)

817 TGATGTTCTGCACCATGGTGTGAACCGTAG788

Wt

108

OF

710 GGATTGTGGCCCCACACAAATACAACCC 737

Co

198

OR

907 CATCAGGCTCGATGGCCAAAACACTCAA 880