Fig. 3

Electrophoresis of agarose gel (2%) of polymerase chain reaction (PCR) product of tetra-primer amplification refractory mutation system- PCR for G6 locus of GDF9 gene. The Lanes of 1 to 11: Amplification of wild type or heterozygous in a mother sheep genomic DNA. Sizes of the common outer product (362 bp) and allele-specific wild type (223 bp) and mutant-type (193 bp) inner product are indicated by an arrow of the respective gel photograph. Lane M: DNA molecular weight marker (O` Range Ruler 100 bp DNA Ladder, Fermentas, Lithuania). G = wild type allele, A = mutant allele