Fig. 1

Electrophoresis of agarose gel (2%) of polymerase chain reaction (PCR) product of tetra-primer amplification refractory mutation system- PCR for G1 locus of GDF9 gene. The Lanes of 1 to 10: Amplification of wild type or heterozygous in a mother sheep genomic DNA. Sizes of common outer product (396 bp) and allele-specific wild type (247 bp) and mutant-type (205 bp) inner product are indicated by an arrow of the respective gel photograph. Lane M: DNA molecular weight marker (O` Range Ruler 100 bp DNA Ladder, Fermentas, Lithuania). G= wild type allele, A= mutant allele