Fig. 7

a Graph illustrating the aggregation tendency profile of every introduced sequence in the On-Def mature peptide. The amino acid aggregation propensity value a3v (green line) is below the hot-spot threshold HST (blue line) in the graph, denoting the presence of fewer aggregation prone residues. b Graph depicting exclusively the area occupied by the residues involved in a hot spot (normalized by the peptide’s length). Regions 6–10, 17–21, and 23–29 of the mature On-Def sequence are predicted as potential aggregation regions