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Table 1 Effect of Murashige and Skoog (MS) and Gamborg’s B5 (B5) media with diverse concentrations of plant growth regulators, 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (KIN) to regenerate Trachyspermum ammi by zygotic embryos

From: Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

  Treatment (mg/l)   Medium Callusinduction Fresh Weightof callus Dry Weightof callus Height of shoot Regeneration Average numberof shoots
BA NAA 2,4-D KIN   (%) (mg) (mg) (cm) (%)  
0 0.5 0 0 B5 68c 0.47 0/03 7.65 62.7c 4.7b
0 1 0 0 B5 56d 0.41 0.034 7.21 65.5c 4.01bc
0 2 0 0 B5 52d 0.44 0.032 6.97 77.4bc 4.12bc
0.5 0.5 0 0 B5 84b 0.48 0.031 9.54 83.4b 4.23bc
0.5 1 0 0 B5 98a 0.53 0.034 10.23 92.6 a 5.9a
0.5 2 0 0 B5 80bc 0.41 0.029 9.12 89.97b 4.8b
1 0.5 0 0 B5 88b 0.43 0.031 9.93 76.6bc 4.9b
1 1 0 0 B5 74bc 0.37 0.027 9.17 80.75bc 4.54bc
1 2 0 0 B5 44e 0.29 0.018 9.19 68.5c 3.7c
0 0 0.5 0.2 MS 72bc 0.31 0.028 7.76 58.8d 3.87c
0 0 0.5 0.5 MS 64c 0.37 0.021 7.98 56.2d 3.54c
0 0 0.5 1 MS 56d 0.39 0.023 6.74 49de 2.5cd
0 0 1 0.2 MS 96a 0.41 0.029 9.97 82.5b 3.9c
0 0 1 0.5 MS 88b 0.47 0.032 7.54 79.9bc 3.76c
0 0 1 1 MS 78bc 0.31 0.029 6.98 68.2c 1.97d
0 0 2 0.2 MS 52d 0.38 0.021 7.12 61.7d 3.81c
0 0 2 0.5 MS 44e 0.33 0.027 6.34 51.3de 2.87cd
0 0 2 1 MS 31f 0.27 0.018 5.53 41.1e 1.89d
  1. a–fMeans with different superscripts within the same column differ significantly (P ≤ 0.05) using Duncan