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Table 1 Effect of Murashige and Skoog (MS) and Gamborg’s B5 (B5) media with diverse concentrations of plant growth regulators, 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (KIN) to regenerate Trachyspermum ammi by zygotic embryos

From: Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

 

Treatment

(mg/l)

 

Medium

Callusinduction

Fresh Weightof callus

Dry Weightof callus

Height of shoot

Regeneration

Average numberof shoots

BA

NAA

2,4-D

KIN

 

(%)

(mg)

(mg)

(cm)

(%)

 

0

0.5

0

0

B5

68c

0.47

0/03

7.65

62.7c

4.7b

0

1

0

0

B5

56d

0.41

0.034

7.21

65.5c

4.01bc

0

2

0

0

B5

52d

0.44

0.032

6.97

77.4bc

4.12bc

0.5

0.5

0

0

B5

84b

0.48

0.031

9.54

83.4b

4.23bc

0.5

1

0

0

B5

98a

0.53

0.034

10.23

92.6 a

5.9a

0.5

2

0

0

B5

80bc

0.41

0.029

9.12

89.97b

4.8b

1

0.5

0

0

B5

88b

0.43

0.031

9.93

76.6bc

4.9b

1

1

0

0

B5

74bc

0.37

0.027

9.17

80.75bc

4.54bc

1

2

0

0

B5

44e

0.29

0.018

9.19

68.5c

3.7c

0

0

0.5

0.2

MS

72bc

0.31

0.028

7.76

58.8d

3.87c

0

0

0.5

0.5

MS

64c

0.37

0.021

7.98

56.2d

3.54c

0

0

0.5

1

MS

56d

0.39

0.023

6.74

49de

2.5cd

0

0

1

0.2

MS

96a

0.41

0.029

9.97

82.5b

3.9c

0

0

1

0.5

MS

88b

0.47

0.032

7.54

79.9bc

3.76c

0

0

1

1

MS

78bc

0.31

0.029

6.98

68.2c

1.97d

0

0

2

0.2

MS

52d

0.38

0.021

7.12

61.7d

3.81c

0

0

2

0.5

MS

44e

0.33

0.027

6.34

51.3de

2.87cd

0

0

2

1

MS

31f

0.27

0.018

5.53

41.1e

1.89d

  1. a–fMeans with different superscripts within the same column differ significantly (P ≤ 0.05) using Duncan