Fig. 4

a Detection of putative transgenic Trachyspermum ammi by PCR 1–3: putative transgenic plant using LBA4404, 4–6: putative transgenic plants using EHA101; NT: non-transgenic plant; P: positive control (plasmid) W: negative control (deionized water); M:100 bp DNA ladder. b DNA-blot analysis of transformed and non-transformed T. ammi. 1, 2, 5, and 6 transgenic plants; NT: non-transformed plant; P: positive control; M: marker (digestion of total genomic DNA was performed using EcoRI). c Results of Thymol content (mg/g plant dried extract) of transformed and non-transformed T. ammi using HPLC methods. One copy or two copy numbers were determined using DNA-blot analysis