Fig. 3

a Histochemical GUS assay using Trachyspermum ammi leaves, T: Putative transformed plant, NT: non-transformed plant (control); b Results related to PCR analysis of GUS, nptII, VIR G, and RT-PCR analysis. 1–3 putative transgenic plant, P: positive control (plasmid), NT: non-transformed herb, W: negative control, M: 100 bp DNA ladder