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Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Production of polyclonal antibody against the recombinant PirBvp protein of Vibrio parahaemolyticus

Fig. 2

Expression of rPirBvp protein analyzed on SDS-PAGE (a), Western blot (b), and purification analyzed on SDS-PAGE and silver staining (c). a and b Lane M: protein ladder; Lane 1: E. coli BL21(DE3)/pET22b (+IPTG); Lane 2: E. coli BL21(DE3)/pET22b (-IPTG); Lanes 3–5: E. coli BL21(DE3)/pET22b-PirBvp (+IPTG). c Lane M: protein ladder; Lane 1: pre-purified rPirBvp protein; Lanes 2–3: whole cell lysate protein; Lanes 4–5: purified rPirBvp protein

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