Confirmation of “pre-plasmolysis mediated ex-osmosis hypothesis” to obtain shoot bud morphogenesis in Catharanthus roseus

Journal of Genetic Engineering and Biotechnology

Table 3 Alkaloids content in hypertonic pre-plasmolytic treatment solution after pre-plasmolysis of leaves

Pre-plasmolysis treatment (CPW: mannitol)	Duration of pre-plasmolysis (min)	cv. Dhawal		cv. Nirmal
Pre-plasmolysis treatment (CPW: mannitol)	Duration of pre-plasmolysis (min)	Vindoline (mg/kg wt ± SD)	Catharanthine (mg/kg wt ± SD)	Vindoline (mg/kg wt ± SD)	Catharanthine (mg/kg wt ± SD)
13%	15	ND	ND	ND	ND
	30	0.014 ± 0.0001	0.013 ± 0.0001	0.013 ± 0.0001	0.013 ± 0.0001
	60	0.024 ± 0.0002	0.023 ± 0.0003	0.013 ± 0.0002	0.013 ± 0.0002
	90	0.042 ± 0.0004	0.051 ± 0.0004	0.023 ± 0.0002	0.026 ± 0.0003
15%	15	ND	ND	ND	ND
	30	0.022 ± 0.0002	0.023 ± 0.0001	0.016 ± 0.0001	0.014 ± 0.0001
	60	0.040 ± 0.0003	0.032 ± 0.0002	0.022 ± 0.0001	0.014 ± 0.0001
	90	0.050 ± 0.0001	0.033 ± 0.0002	0.024 ± 0.0001	0.022 ± 0.0001
20%	15	0.012 ± 0.0001	0.014 ± 0.0001	0.014 ± 0.0002	0.012 ± 0.0001
	30	0.022 ± 0.0002	0.022 ± 0.0002	0.016 ± 0.0001	0.013 ± 0.0001
	60	0.034 ± 0.0003	0.025 ± 0.0002	0.021 ± 0.0002	0.021 ± 0.0001
	90	0.052 ± 0.0004	0.026 ± 0.0003	0.033 ± 0.0003	0.025 ± 0.0002

Data expressed as the mean ± standard deviation (SD) of the three independent biological replicates with P < 0.005; ND not detected