Table 2 Effect of pre-plasmolysis treatment on direct shoot bud regeneration response from leaf explants cultured on shoot regeneration media

0%

0

14 ± 1.2

12 ± 1.2

1.11 ± 0.01

1.14 ± 0.05

60–65

50–60

1%

15

40 ± 3.6

17 ± 1.6

1.16 ± 0.03

1.07 ± 0.04

55–60

55–60

30

66 ± 7.2

27 ± 2.4

1.05 ± 0.05

1.09 ± 0.05

55–60

55–60

60

70 ± 7.4

37 ± 3.4

1.13 ± 0.07

1.09 ± 0.05

45–50

45–50

90

-

-

-

-

-

-

10%

15

33 ± 3.6

27 ± 2.4

1.21 ± 0.07

1.13 ± 0.07

45–50

45–50

30

43 ± 4.4

37 ± 3.2

1.15 ± 0.05

1.09 ± 0.07

40–45

40–45

60

-

-

-

-

-

-

90

-

-

-

-

-

-

13%

15

67 ± 7.2

27 ± 2.4

1.63 ± 0.12

1.28 ± 0.09

35–40

35–40

30

83 ± 9.1

43 ± 4.6

1.46 ± 0.09

1.31 ± 0.11

35–40

35–40

60

-

-

-

-

-

-

90

-

-

-

-

-

-

15%

15

60 ± 6.8

23 ± 2.2

1.16 ± 0.07

1.43 ± 0.13

30–35

30–35

30

77 ± 8.2

40 ± 4.2

1.26 ± 0.09

1.33 ± 0.11

30–35

30–35

60

-

-

-

-

-

-

90

-

-

-

-

-

-

20%

15

1.2 ± 0.05

1.0 ± 0.03

30–35

30–35

30

-

-

-

-

-

-

60

-

-

-

-

-

-

90

-

-

-

-

-

-