Table 1 Primers used for cloning and PCR
From: Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein
Gene construct | Primer name | Primer sequence 5′-3′ |
|---|---|---|
GVAC08 | V04FP | CCATGGGGATGGCGAGCGCGAG |
V08RP | CTCGAGGCTCAGGCTCGGGGTACCG | |
T7 forward | TAATACGACTCACTATAGGG | |
T7 reverse | GCTAGTTATTGCTCAGCGG |