Skip to main content

Table 5 Relative rate of hydrolysis of various substrates by Aspergillus sp. DHE7 β-glucosidase

From: Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation

Substrate Relative activity/%a
Aryl glycosides
 p-Nitrophenyl-β-D-glucopyranoside 100b
 p-Nitrophenyl-α-D-glucopyranoside 0.0
 p-Nitrophenyl-β-D-galactopyranoside 13
 p-Nitrophenyl-α-D-galactopyranoside 0.0
 Cellobiose 100c
 Lactose 18
 Maltose 0.0
 Salicin 72
 Sucrose 0.0
 CMC 0.0
 Starch 0.0
  1. aThe relative hydrolytic rate was determined as percentage of that obtained with p-NPG for p-NP substrates and percentage of that obtained with cellobiose for di- and polysaccharides
  2. bRelative hydrolytic activity of enzyme on p-NP substrates was calculated by released p-NP concentration
  3. cRelative hydrolytic activity of enzyme on oligo- and polysaccharides was calculated by analyzing the released glucose concentration using DNS method