Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation

Journal of Genetic Engineering and Biotechnology

Table 5 Relative rate of hydrolysis of various substrates by Aspergillus sp. DHE7 β-glucosidase

Substrate	Relative activity/%^a
Aryl glycosides
p-Nitrophenyl-β-D-glucopyranoside	100^b
p-Nitrophenyl-α-D-glucopyranoside	0.0
p-Nitrophenyl-β-D-galactopyranoside	13
p-Nitrophenyl-α-D-galactopyranoside	0.0
Oligosaccharides
Cellobiose	100^c
Lactose	18
Maltose	0.0
Salicin	72
Sucrose	0.0
Polysaccharides
CMC	0.0
Starch	0.0

^aThe relative hydrolytic rate was determined as percentage of that obtained with p-NPG for p-NP substrates and percentage of that obtained with cellobiose for di- and polysaccharides
^bRelative hydrolytic activity of enzyme on p-NP substrates was calculated by released p-NP concentration
^cRelative hydrolytic activity of enzyme on oligo- and polysaccharides was calculated by analyzing the released glucose concentration using DNS method