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Table 5 Actual data for design of experiments

From: Antioxidant potential and optimization of production of extracellular polysaccharide by Acinetobacter indicus M6

S.No.

Glucose

Yeast extract

MgSO4

Production of biosurfactant in g/L

Experimentala

Predictedb

1.

15

10

0.3

1.95

2.1

2.

5

15

0.4

0.36

0.39

3.

10

10

0.3

2.51

2.54

4.

15

15

0.2

1.08

1.03

5.

5

5

0.4

0.63

0.64

6.

10

10

0.3

2.54

2.54

7.

10

10

0.4

2.36

2.34

8.

10

10

0.3

2.63

2.54

9.

10

5

0.3

1.22

1.34

10.

15

5

0.4

0.66

0.63

11.

10

15

0.3

1.25

1.28

12.

5

15

0.2

0.46

0.45

13.

10

10

0.3

2.65

2.54

14.

15

5

0.2

0.98

0.91

15.

10

10

0.3

2.56

2.54

16.

5

10

0.3

1.8

1.81

17.

10

10

0.3

2.65

2.54

18.

5

5

0.2

0.42

0.38

19.

15

15

0.4

0.44

0.44

20.

10

10

0.2

2.34

2.51

  1. aExperimental result of biosurfactant production at the mentioned nutrient concentrations
  2. bRSM predicted value of biosurfactant production at the mentioned nutrient concentrations