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Table 6 Effect of different cytokinins and activated charcoal on secondary embryo number, shoot number, and vitrified embryos after 3 recultures

From: High-frequency direct somatic embryogenesis and plantlet regeneration from date palm immature inflorescences using picloram

Treatments (mg/l)

Secondary embryo n.

Shoot number

Vitrified embryos n

With AC

Without AC

Mean

With AC

Without AC

Mean

With AC

Without AC

Mean

Control (without cytokinin)

6.20 h

11.10 g

8.65 E

11.60 ef

9.42 f

10.51D

3.50 e

0.00 f

1.75 D

0.25 BA

39.00 c

48.66 a

43.83A

22.00bc

19.60 cd

20.80B

6.80 cd

7.20 c

7.00 B

0.25 Kinetin

26.00 e

36.50 c

31.25C

29.00 a

24.20 b

26.60A

4.55 de

5.60 cde

5.07 C

0.25 2ip

16.30 f

25.20 e

20.75D

17.00 d

13.50 e

15.25C

15.00 b

17.90 a

16.45 A

0.1 2ip + 0.1 kinetin + 0.1 BA

31.70 d

43.50 b

37.60B

23.00 b

18.30 d

20.65B

5.20 cde

7.50 c

6.35 BC

Mean

23.84 B

32.99 A

 

20.52 A

17.00 B

 

7.01 A

7.64 A

 
  1. Means with different letters for each parameter are significantly different at 5%