Fig. 2

Western blotting analysis of the recombinant antiEpEX-scFv protein. Bacterial lysates of four E. coli strains including BL21^TM (DE3), SHuffle^TM T7, Origami^TM (DE3), and Rosetta^TM (DE3) before induction (un) and after induction were treated with the anti His tag polyclonal antibody The positive control was E. coli K-12 BW25113 cells harboring the corresponding plasmid and were inducted by 0.8 mM IPTG at 37 °C for 24 h (C+)