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Table 4 List of selected ISSR primers and their codes, sequences, annealing temperature, the number of amplified markers, and percentage of polymorphism for each primer in Salvadora persica samples treated with Fe3O4, SiO2, or ZnO nanoparticles and their controls

From: Nanoparticles induce genetic, biochemical, and ultrastructure variations in Salvadora persica callus

No.

Primer code

Sequencing

(5′-3′)

Annealing Temp.

Total band

Monomorphic band

Polymorphic band

Unique

Polymorphism%

+ve

-ve

1

17898A

(CA)6AC

49

16

6

10

1

2

62.5

2

17899A

(CA)6AG

49

12

4

8

3

2

66.6

3

17898B

(CA)6GT

50

16

2

14

7

7

87.5

4

17899B

(CA)6GG

50

8

4

4

0

1

50

5

UBC807

(AG)8GT

52

14

2

12

5

4

85.7

6

UBC808

(AG)8GC

52

11

3

8

1

3

72.7

7

UBC810

(GA)8T

52

10

3

7

4

2

70

8

UBC811

(GA)8AC

52

23

1

22

7

5

95.6

9

UBC814

(CT)8A

52

12

1

11

0

3

91.6

10

UBC824

(TC)8G

52

8

5

3

0

0

37.5

11

UBC826

(AC)8C

52

13

0

13

2

0

100

12

UBC828

(TG)8A

52

15

2

13

2

0

86.6

13

UBC862

(AGC)6

52

8

5

3

1

0

37.5

14

UBC864

(ATG)6

52

14

1

13

5

1

92.8

15

UBC873

(GACA)4

52

11

1

10

6

2

90.9

 

Total

  

191

40

151

44

32

79.1