Table 3 Central composite design (CCD) matrix by the three tested variables besides the observed and expected amylase activity exerted by the model trials
Run | Coded parameters | Mean amylase activity (U/mL/min) | |||
|---|---|---|---|---|---|
A | B | C | Observed | Expected | |
1 | 8.0 | 50.0 | 96.0 | 0 | 12.9 |
2 | 6.5 | 62.5 | 60.0 | 65 | 63.3 |
3 | 6.5 | 41.4 | 60.0 | 24.4 | 30.8 |
4 | 6.5 | 62.5 | 60.0 | 65.5 | 63.3 |
5 | 3.9 | 62.5 | 60.0 | 0 | 19.1 |
6 | 6.5 | 62.5 | 60.0 | 62.3 | 63.3 |
7 | 5.0 | 50.0 | 96.0 | 80.7 | 71.2 |
8 | 5.0 | 75.0 | 24.0 | 3.9 | − 12.5 |
9 | 5.0 | 50.0 | 24.0 | 32.2 | 20.9 |
10 | 8.0 | 75.0 | 24.0 | 0 | 6.1 |
11 | 5.0 | 75.0 | 96.0 | 18.2 | 20.6 |
12 | 9.0 | 62.5 | 60.0 | 0 | − 14.2 |
13 | 8.0 | 50.0 | 24.0 | 0 | − 5.9 |
14 | 6.5 | 62.5 | 120.5 | 68.3 | 58.5 |
15 | 6.5 | 62.5 | 60.0 | 62.2 | 63.3 |
16 | 8.0 | 75.0 | 96.0 | 0 | 7.8 |
17 | 6.5 | 62.5 | 0.0 | 0 | 14.7 |
18 | 6.5 | 62.5 | 60.0 | 62.9 | 63.3 |
19 | 6.5 | 83.5 | 60.0 | 0 | − 1.6 |
20 | 6.5 | 62.5 | 60.0 | 62.9 | 63.3 |