Table 2 The full factorial design matrix showing the observed amylase activity of P. thermoglucosidasius Pharon1 exerted by the design trails
Run | Coded parameters | Mean amylase activity (U/mL/min) | ||||
|---|---|---|---|---|---|---|
A | B | C | D | Observed | Expected | |
1 | 8 | 50 | 96 | Aerobic | 0.0 | 0.3 |
2 | 5 | 50 | 96 | Anaerobic | 18.2 | 18.5 |
3 | 8 | 50 | 24 | Anaerobic | 0.0 | 0.3 |
4 | 5 | 75 | 96 | Anaerobic | 6.1 | 5.8 |
5 | 8 | 50 | 24 | Aerobic | 0.0 | − 0.3 |
6 | 5 | 75 | 24 | Anaerobic | 2.1 | 2.4 |
7 | 8 | 75 | 96 | Anaerobic | 0.0 | 0.3 |
8 | 5 | 50 | 24 | Anaerobic | 5.0 | 4.7 |
9 | 8 | 75 | 96 | Aerobic | 0.0 | − 0.3 |
10 | 5 | 75 | 96 | Aerobic | 17.4 | 17.7 |
11 | 5 | 50 | 24 | Aerobic | 32.0 | 32.3 |
12 | 8 | 75 | 24 | Aerobic | 0.0 | 0.3 |
13 | 8 | 75 | 24 | Anaerobic | 0.0 | − 0.3 |
14 | 5 | 50 | 96 | Aerobic | 59.4 | 59.1 |
15 | 8 | 50 | 96 | Anaerobic | 0.0 | − 0.3 |
16 | 5 | 75 | 24 | Aerobic | 3.3 | 3.0 |