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Table 1 A typical purification scheme of Egyptian honey bee venom PLA2

From: Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities

Purification steps

Total protein

(mg)

Total

activity

(unit)

Specific

activity

Yield

(%)

Fold

purification

Crude bee venom

47.2

31875

675

100

1

DEAE-cellulose major peak

9.3

20763

2234

65

3.3

Sephacryl S-300 fraction

2.0

12066

6033

38

8.9