Fig. 5
From: Paper-based PCR method development, validation and application for microbial detection
PCR master mix volume variation. a 16s r-DNA: lane 1—100 bp ladder, lane 2—colony PCR, lane 3—25 μL master mix volume, lane 4—50 μL master mix volume, lane 5—negative control. b β-galactocidase lane 1—100 bp ladder, lane 2—colony PCR, lane 3—25 μL master mix volume, lane 4—50 μL master mix volume, lane 5—negative control. c ITS: lane 1—100 bp ladder, lane 2—colony PCR, lane 3—25 μL master mix volume, lane 4—50 μL master mix volume, lane 5—negative control