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Table 1 Repeatability and reproducibility of HER-2 Ille655Val real-time assay

From: Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer

Genotype

Intra-run assay (Ct)

Inter-run assay (Ct)

Day 1

Day 2

Day 3

CV

SD

CV

SD

CV

SD

CV

AA

0.22

1.17

0.17

0.908

0.043

0.22

0.77

AG

0.23

1.041

0.136

0.6

0.06

0.26

0.78

GG

0.26

1.32

0.122

0.62

0.125

0.65

1.09

  1. SD standard deviation, CV coefficient of variability