Table 4 Cleavage of amino acid residues by different enzymes generated by ExPASy peptide cutter

Arg-C proteinase

8

36, 48, 91, 183, 204, 221, 270, 286

Asp-N endopeptidase

23

15, 32, 43, 57, 59, 67, 72, 82, 95, 124, 126, 138, 170, 180, 185, 191, 200, 207, 213, 215, 226, 261, 294

Asp-N endopeptidase + N-terminal Glu

31

15, 32, 43, 57, 59, 67, 72, 82, 95, 124, 126, 138, 143, 150, 160, 166, 170, 177, 180, 185, 187, 190, 191, 200, 207, 213, 215, 226, 261, 294, and 302

BNPS-Skatole

3

56, 116, and 187

CNBr

2

121 and 206

Caspase1

1

58

Chymotrypsin-high specificity

37

29, 30, 41, 43, 56, 63, 67, 76, 77, 82, 84, 85, 89, 107, 111, 115, 116, 123, 131, 158, 173, 179, 180, 187, 194, 212, 218, 222, 243, 252, 268, 269, 275, 276, 282, 297, and 311

Chymotrypsin-low specificity

62

14, 20, 24, 29, 30, 31, 41, 43, 55, 56, 63, 67, 75, 76, 77, 82, 84, 85, 89, 101, 106, 107, 111, 115, 116, 121, 123, 131, 134, 143, 145, 147, 156, 158, 165, 173, 176, 179, 180, 187, 194, 203, 206, 212, 217, 218, 222, 232, 243, 244, 245, 251, 252, 264, 268, 269, 275, 276, 282, 296, 297, and 311

Clostripain

8

36, 48, 91, 183, 204, 221, 270, and 286

Formic acid

23

16, 33, 44, 58, 60, 68, 73, 83, 96, 125, 127, 139, 171, 181, 186, 192, 201, 208, 214, 216, 227, 262, and 295

Glutamyl endopeptidase

8

144, 151, 161, 167, 178, 188, 191, and 303

Hydroxylamine

3

117, 228, and 247

Iodosobenzoic acid

3

56, 116, and 187

LysC

14

6, 11, 18, 46, 80, 86, 102, 198, 211, 220, 240, 261, 263, and 308

LysN

14

5, 10, 17, 45, 79, 85, 101, 197, 210, 219, 239, 260, 262, and 307

Pepsin (pH 1.3)

45

14, 19, 23, 24, 30, 31, 40, 41, 51, 54, 55, 62, 63, 89, 100, 114, 115, 130, 131, 133, 134, 144, 155, 156, 164, 165, 172, 173, 175, 176, 178, 179, 202, 203, 243, 244, 245, 264, 275, 276, 281, 282, 295, 296, and 311

Pepsin (pH > 2)

81

14, 19, 23, 24, 28, 29, 30, 31, 40, 41, 42, 43, 51, 54, 55, 56, 62, 63, 66, 67, 75, 76, 81, 83, 84, 85, 89, 100, 106, 107, 110, 111, 114, 115, 116, 122, 123, 130, 131, 133, 134, 144, 155, 156, 157, 158, 164, 165, 172, 173, 175, 176, 178, 179, 180, 187, 193, 202, 203, 211, 212, 217, 218, 243, 244, 245, 251, 252, 264, 267, 268, 269, 274, 275, 276, 281, 282, 295, 296, 297, and 311

Proteinase K

159

1, 2, 4, 7, 9, 13, 14, 17, 20, 22, 23, 24, 29, 30, 31, 35, 38, 39, 40, 41, 42, 43, 45, 50, 51, 54, 55, 56, 57, 59, 62, 63, 65, 66, 67, 69, 70, 71, 72, 74, 76, 77, 78, 81, 82, 84, 85, 87, 88, 89, 94, 97, 99, 101, 104, 105, 107, 111, 114, 115, 116, 122, 123, 129, 130, 131, 132, 134, 138, 140, 141, 144, 145, 146, 148, 149, 150, 151, 156, 157, 158, 161, 164, 165, 167, 168, 169, 172, 173, 175, 176, 177, 178, 179, 180, 187, 188, 189, 191, 193, 194, 195, 199, 202, 203, 210, 212, 218, 222, 223, 231, 233, 237, 238, 242, 243, 244, 245, 246, 250, 252, 254, 255, 256, 257, 259, 264, 266, 267, 268, 269, 271, 273, 275, 276, 277, 280, 281, 282, 285, 287, 289, 290, 292, 293, 294, 296, 297, 299, 302, 303, 304, 305, 306, 307, 311, 313, 314, and 316

Staphylococcal peptidase I

8

144, 151, 161, 167, 178, 188, 191, and 303

Thermolysin

78

6, 12, 13, 19, 23, 30, 37, 39, 40, 54, 56, 61, 62, 64, 65, 69, 70, 71, 77, 86, 88, 93, 100, 104, 113, 114, 120, 121, 128, 130, 133, 137, 140, 147, 148, 155, 156, 163, 164, 168, 172, 175, 176, 198, 202, 205, 230, 236, 237, 241, 243, 244, 245, 253, 255, 258, 263, 265, 266, 270, 275, 281, 284, 286, 288, 289, 291, 292, 293, 298, 301, 304, 305, 306, 310, 312, 313, and 315

Trypsin

22

6, 11, 18, 36, 46, 48, 80, 86, 91, 102, 183, 198, 204, 211, 220, 221, 240, 261, 263, 270, 286, and 308