Table 5 Identification of MSpI cutting sites and fragment sizes for genes encoding for alcohol production body and promoter regions of S. cerevisiaea S288C and S. pombe 972h-
S. Cerevisiaea S288C | S. pombe 972h- | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Gene body | Promoter regions | Sequence name | Gene body | Promoter regions | |||||
Sequence name | No. of cut sites (cut position/s) | Fragment size | No. of cut sites (cut position/s) | Fragment size | No. of cut sites (cut position/s) | Fragment size | No. of cut sites (cut position/s) | Fragment size | |
ADH1 | 1 (632) | – | adh1 | 2 (644, 743) | 99 | ||||
ADH3 | 0 | – | 1 (827) | – | adh4 | 0 | – | 3 (71, 501, 570) | 69 |
ADH4 | 1 (146) | – | 2 (276, 650) | – | SPBC1773.06c | 1 (940) | – | 0 | – |
ADH5 | 2 (88, 539) | – | 1 (827) | – | prl | 1 (289) | – | 0 | – |
ADH6 | 1 (992) | – | 2 (271, 979) | – | SPBC8E4.04 | 0 | – | 1 (736) | – |
ADH7 | 0 | – | 1 (507) | – | SPCC13B11.04c | 1 (553) | – | 3 (95, 316, 506) | – |
AAD10 | 0 | – | 1 (489) | – | SPAC19G12.09 | 1(401) | – | 0 | – |
AAD14 | 3 (74, 314, 991) | – | 1 (440) | – | SPAC2E1P3.01 | 1 (825) | – | 0 | – |
AAD15 | 1 (322) | – | 0 | – | Yak3 | 1 (511) | – | 0 | – |
AAD3 | 0 | – | 0 | – | SPAP32A8.02 | 1 (353) | – | 2 (886, 933) | 47 |
AAD4 | 2 (176, 928) | – | 2 (236, 902) | – | SPAC26F1.07 | 2 (74, 941) | – | 0 | – |
AAD6 | 1 (1065) | – | 1 (428) | – | erg27 | 2 (29, 787) | – | 0 | – |
ARA1 | 2 (307, 454) | 147 | 0 | – | SPBC16A3.02c | 2 (238, 431) | 193 | 0 | – |
BDH2 | 3 (315, 481, 806) | 166 | 2 (411, 918) | – | SPBC337.11 | 2 (331, 577) | – | 2 (855, 948) | 93 |
GCY1 | 2 (697, 737) | 40 | 2 (198, 977) | – | SPAC977.14c | 0 | – | 1 (739) | – |
GRE3 | 1 (456) | – | 2 (331, 496) | 185 | SPCC1281.04 | 0 | – | 0 | – |
SFA1 | 2 (221, 326) | 105 | 1 (273) | – | SPAC750.01 | 0 | – | 0 | – |
YFL041W–A | 0 | – | 1 (768) | – | SPAC2F3.05c | 0 | – | 0 | – |
YKL071W | 1 (550) | – | 1 (142) | – | SPAC3A11.11c | 1 (809) | – | 1 (942) | – |
YPR127W | 1 (868) | – | 0 | – | SPBC215.11c | – | 0 | – | |
YPR1 | 2 (206, 268) | 62 | 1 (81) | – | – | ||||