Substrate | Vmax (U/mg/min) | Km (mM) | Kcat (S−1) | Kcat/Km (1/mM S) |
---|
oNPG | 14.2 | 1.4 | 312 | 219.7 |
Lactose | 6.4 | 12.92 | 93 | 7.1 |
- Enzyme activity for the selected substrates was assessed under standard conditions using 0.5–25 mM of O-nitrophenyl b-d-galactopyranoside or 20–360 mM of lactose in 50 mM sodium phosphate buffer (pH 7.8) at 40 °C for 10 min. For lactose-hydrolyzing activity, glucose oxidase was used to determine the specific activity. The Km and Vmax values were calculated by a Lineweaver-Burk plot