Skip to main content

Table 1 Screening of β-galactosidase activity

From: Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Trans-conjugants

Protein

(mg/ml)

Enzyme activity

(U/ml)

Specific activity

(U/mg)

Tra5

3.43 ± 0.16

109.33 ± 2.05

31.9 ± 2.19

Tra10

3.36 ± 0.047

101.83 ± 0.97

30.2 ± 0.37

Tra127

5.3 ± 0.08

71.33 ± 1.47

13.4 ± 0.14

Tra200

2.76 ± .047

31.26 ± 0.89

10.92 ± 0.22

Tra202

3.53 ± 0.094

41.3 ± 0.90

11.69 ± 0.54

Tra210

1.8 ± 0.081

77 ± 1.41

41.96 ± 0.97

Tra212

5.03 ± 0.094

90.93 ± 1.03

18.03 ± 0.32

Tra213

3.6 ± 0.16

40.47 ± 0.42

11.02 ± 0.24

Tra216

4.26 ± 0.094

110.97 ± 0.56

25.96 ± 0.47

Tra222

3.03 ± 0.12

100.55 ± 0.25

33.2 ± 1.41

Tra230

5.73 ± 0.092

81.08 ± 0.72

14.1 ± 0.16

Tra233

2.2 ± 0.081

20.9 ± 0.08

9.46 ± 0.32

Tra234

2.83 ± 0.047

30.77 ± 0.67

10.8 ± 0.40

Tra250

4.13 ± .091

40.54 ± 0.51

9.81 ± 0.24

Tra252

5.63 ± 0.047

70.92 ± 0.52

12.57 ± 0.2

Tra257

3.46 ± 0.091

111.36 ± 0.90

32.1 ± 0.88

Tra258

2.2 ± 0.081

19.81 ± 0.39

8.98 ± 0.43

Tra271

4.33 ± 0.047

51.41 ± 0.83

11.8 ± 0.16