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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Fig. 3

Biochemical characterizations of the trans-mutant E. coli DH5α LacZ. a Effect of temperatures (30–80 °C) on the enzyme activity carried out at pH 6.5 and 5 mM of oNPG. b Effect of pH on the enzyme activity and stability using different buffers (pH 4–10) at 40 °C and 5 mM of oNPG. c Effect of the ionic strength on the enzyme activity using NaCl (0.25–8 M) at 40 °C, pH 7.5 and 5 mM oNPG. d Thermal stability of the enzyme carried out at different temperatures (40–90 °C). e Enzyme stability at 4 °C, pH 7.5 and 5 mM oNPG

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