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Journal of Genetic Engineering and Biotechnology

Table 1 Partial purification of lipase from R. oryzae R1 by fractional precipitation of ammonium sulfate

From: Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Ammonium sulfate saturation %

Lipase activity (U/mL)

Protein content (mg/mL)

Specific activity (U/mg protein)

Yield (%)

Purification fold

Crude extract

210.0

150.0

1.40

100.0

1.00

Fraction (I) 0–20%

191.5

129.5

1.48

91.2

1.05

Fraction (II) 20–40%

183.8

118.3

1.56

87.5

1.11

Fraction (III) 40–60%

161.2

158.0

1.02

76.8

0.72

Fraction (IV) 60–80%

137.5

163.0

0.84

65.5

0.60

  1. Specific activity (measures the enzyme’s purity) = (enzyme activity/protein content); yield (measures enzyme’s activity preservation) = (retained activity x 100/initial activity); and purification fold = (specific activity/initial specific activity)
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