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Table 1 Partial purification of lipase from R. oryzae R1 by fractional precipitation of ammonium sulfate

From: Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Ammonium sulfate saturation % Lipase activity (U/mL) Protein content (mg/mL) Specific activity (U/mg protein) Yield (%) Purification fold
Crude extract 210.0 150.0 1.40 100.0 1.00
Fraction (I) 0–20% 191.5 129.5 1.48 91.2 1.05
Fraction (II) 20–40% 183.8 118.3 1.56 87.5 1.11
Fraction (III) 40–60% 161.2 158.0 1.02 76.8 0.72
Fraction (IV) 60–80% 137.5 163.0 0.84 65.5 0.60
  1. Specific activity (measures the enzyme’s purity) = (enzyme activity/protein content); yield (measures enzyme’s activity preservation) = (retained activity x 100/initial activity); and purification fold = (specific activity/initial specific activity)