Table 2 Putative hydrocarbon degradation genes detected in the FN1 metagenome and their taxonomic affiliation
Hydrocarbon degradation genes | Taxonomic affiliation |
|---|---|
Degradation of aromatic compounds | |
 ko:K00001 E1.1.1.1; alcohol dehydrogenase [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K01055 pcaD; 3-oxoadipate enol-lactonase [EC:3.1.1.24] | Mesorhizobium albiziae |
 ko:K04073 mhpF; acetaldehyde dehydrogenase [EC:1.2.1.10] | Pelagibacterium sp. SCN 64-44 |
 ko:K05714 mhpC; 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase [EC:3.7.1.14] | Mesorhizobium albiziae |
 ko:K05783 benD-xylL; dihydroxycyclohexadiene carboxylate dehydrogenase [EC:1.3.1.25 1.3.1.-] | Rhizobiales bacterium |
 ko:K05784 benC-xylZ; benzoate/toluate 1,2-dioxygenase reductase component [EC:1.18.1.-] | Rhizobiales bacterium |
 ko:K07535 badH; 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase [EC:1.1.1.-] | Candidatus Rokubacteria bacterium; Mesorhizobium denitrificans; Rhizobiales bacterium |
 ko:K07538 had; 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase [EC:1.1.1.368] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K07545 bbsG; (R)-benzylsuccinyl-CoA dehydrogenase [EC:1.3.8.3] | Nocardioides sp. dk3543 |
 ko:K07547 bbsC; 2-[hydroxy(phenyl)methyl]-succinyl-CoA dehydrogenase BbsC subunit [EC:1.1.1.35] | Rhizobiales bacterium |
 ko:K07548 bbsD; 2-[hydroxy(phenyl)methyl]-succinyl-CoA dehydrogenase BbsD subunit [EC:1.1.1.35] | Rhizobiales bacterium |
 ko:K10216 dmpD; 2-hydroxymuconate-semialdehyde hydrolase [EC:3.7.1.9] | Mesorhizobium albiziae |
 ko:K10219 ligC; 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase [EC:1.1.1.312] | Pelagibacterium sp. SCN 64-44; Devosia sp. LC5; Devosia elaeis |
 ko:K10222 bphD; 2,6-dioxo-6-phenylhexa-3-enoate hydrolase [EC:3.7.1.8] | Mesorhizobium albiziae |
 ko:K10617 cymB; p-cumic alcohol dehydrogenase | Rhizobiales bacterium |
 ko:K10620 cmtB; 2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase [EC:1.3.1.58] | Rhizobiales bacterium |
 ko:K13953 adhP; alcohol dehydrogenase, propanol-preferring [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K14727 pcaL; 3-oxoadipate enol-lactonase / 4-carboxymuconolactone decarboxylase [EC:3.1.1.24 4.1.1.44] | Mesorhizobium albiziae |
 ko:K14746 ped; (S)-1-phenylethanol dehydrogenase [EC:1.1.1.311] | Rhizobiales bacterium |
 ko:K15571 bnsG; naphthyl-2-methylsuccinyl-CoA dehydrogenase [EC:1.3.99.-] | Nocardioides sp. dk3543 |
 ko:K17754 cpnA; cyclopentanol dehydrogenase [EC:1.1.1.163] | Rhizobiales bacterium |
 ko:K18067 pht4; phthalate 4,5-cis-dihydrodiol dehydrogenase [EC:1.3.1.64] | Pelagibacterium sp. SCN 64-44; Devosia sp. LC5; Devosia elaeis |
 ko:K18092 etbD; 2-hydroxy-6-oxo-octa-2,4-dienoate hydrolase [EC:3.7.1.-] | Mesorhizobium albiziae |
 ko:K19960 chnA; cyclohexanol dehydrogenase [EC:1.1.1.245] | Rhizobiales bacterium |
Benzoate degradation | |
 ko:K01055 pcaD; 3-oxoadipate enol-lactonase [EC:3.1.1.24] | Mesorhizobium albiziae |
 ko:K04073 mhpF; acetaldehyde dehydrogenase [EC:1.2.1.10] | Pelagibacterium sp. SCN 64-44 |
 ko:K04117 aliB; cyclohexanecarboxyl-CoA dehydrogenase [EC:1.3.99.-] | Nocardioides sp. |
 ko:K04118 E1.3.1.62; pimeloyl-CoA dehydrogenase [EC:1.3.1.62] | Nocardioides sp. |
 ko:K05783 benD-xylL; dihydroxycyclohexadiene carboxylate dehydrogenase [EC:1.3.1.25 1.3.1.-] | Rhizobiales bacterium |
 ko:K05784 benC-xylZ; benzoate/toluate 1,2-dioxygenase reductase component [EC:1.18.1.-] | Rhizobiales bacterium |
 ko:K07535 badH; 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase [EC:1.1.1.-] | Candidatus Rokubacteria bacterium; Mesorhizobium denitrificans; Rhizobiales bacterium |
 ko:K07538 had; 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase [EC:1.1.1.368] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K10216 dmpD; 2-hydroxymuconate-semialdehyde hydrolase [EC:3.7.1.9] | Mesorhizobium albiziae |
 ko:K10219 ligC; 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase [EC:1.1.1.312] | Pelagibacterium sp. SCN 64-44; Devosia sp. LC5; Devosia elaeis |
 ko:K14334 CMLE; carboxy-cis,cis-muconate cyclase [EC:5.5.1.5] | Mesorhizobium sp.; Mesorhizobium prunaredense; Mesorhizobium sp. M2A.F.Ca.ET.042.01.1.1; |
 ko:K14727 pcaL; 3-oxoadipate enol-lactonase / 4-carboxymuconolactone decarboxylase [EC:3.1.1.24 4.1.1.44] | Mesorhizobium albiziae |
 ko:K16173 acd; glutaryl-CoA dehydrogenase (non-decarboxylating) [EC:1.3.99.32] | Nocardioides sp. dk3543 |
 ko:K19066 E1.3.8.10; cyclohex-1-ene-1-carbonyl-CoA dehydrogenase [EC:1.3.8.10] | Nocardioides sp. dk3543 |
 ko:K19067 E1.3.8.11; cyclohexane-1-carbonyl-CoA dehydrogenase [EC:1.3.8.11] | Nocardioides sp. dk3543 |
Xylene degradation | |
 ko:K04073 mhpF; acetaldehyde dehydrogenase [EC:1.2.1.10] | Pelagibacterium sp. SCN 64-44 |
 ko:K05783 benD-xylL; dihydroxycyclohexadiene carboxylate dehydrogenase [EC:1.3.1.25 1.3.1.-] | Rhizobiales bacterium |
 ko:K05784 benC-xylZ; benzoate/toluate 1,2-dioxygenase reductase component [EC:1.18.1.-] | Rhizobiales bacterium |
 ko:K10216 dmpD; 2-hydroxymuconate-semialdehyde hydrolase [EC:3.7.1.9] | Mesorhizobium albiziae |
 ko:K10617 cymB; p-cumic alcohol dehydrogenase | Rhizobiales bacterium |
 ko:K10620 cmtB; 2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase [EC:1.3.1.58] | Rhizobiales bacterium |
Chlorocyclohexane and chlorobenzene degradation | |
 ko:K01561 dehH; haloacetate dehalogenase [EC:3.8.1.3] | Mesorhizobium albiziae |
 ko:K01563 dhaA; haloalkane dehalogenase [EC:3.8.1.5] | Mesorhizobium albiziae |
 ko:K15237 linC; 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase 1 [EC:1.3.1.-] | Mesorhizobium denitrificans; Rhizobiales bacterium |
 ko:K15238 linX; 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase 2 [EC:1.3.1.-] | Rhizobiales bacterium |
 ko:K15241 pcpC; tetrachlorohydroquinone reductive dehalogenase [EC:1.21.4.5] | Devosia sp. I507 |
Chloroalkane and chloroalkene degradation | |
 ko:K00001 E1.1.1.1; alcohol dehydrogenase [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K01561 dehH; haloacetate dehalogenase [EC:3.8.1.3] | Mesorhizobium albiziae |
 ko:K01563 dhaA; haloalkane dehalogenase [EC:3.8.1.5] | Mesorhizobium albiziae |
 ko:K13953 adhP; alcohol dehydrogenase, propanol-preferring [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
Toluene degradation | |
 ko:K07545 bbsG; (R)-benzylsuccinyl-CoA dehydrogenase [EC:1.3.8.3] | Nocardioides sp. dk3543 |
 ko:K07547 bbsC; 2-[hydroxy(phenyl)methyl]-succinyl-CoA dehydrogenase BbsC subunit [EC:1.1.1.35] | Rhizobiales bacterium |
 ko:K07548 bbsD; 2-[hydroxy(phenyl)methyl]-succinyl-CoA dehydrogenase BbsD subunit [EC:1.1.1.35] | Rhizobiales bacterium |
 ko:K19630 tsaC1; 4-formylbenzenesulfonate dehydrogenase [EC:1.2.1.62] | Rhizobiales bacterium |
Naphthalene degradation | |
 ko:K00001 E1.1.1.1; alcohol dehydrogenase [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K13953 adhP; alcohol dehydrogenase, propanol-preferring [EC:1.1.1.1] | Mesorhizobium denitrificans; Microbacterium lacticum |
 ko:K15571 bnsG; naphthyl-2-methylsuccinyl-CoA dehydrogenase [EC:1.3.99.-] | Nocardioides sp. dk3543 |
Aminobenzoate degradation | |
 ko:K09461 E1.14.13.40; anthraniloyl-CoA monooxygenase [EC:1.14.13.40] | Azospirillum sp. TSA6c; Azospirillum doebereinerae |
 ko:K10219 ligC; 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase [EC:1.1.1.312] | Pelagibacterium sp. SCN 64-44; Devosia sp. LC5; Devosia elaeis |
 ko:K22959 lpdB; gallate decarboxylase subunit B | Pseudaminobacter sp. CB3; Hyphomicrobiaceae bacterium TMED74 |
Ethylbenzene degradation | |
 ko:K14746 ped; (S)-1-phenylethanol dehydrogenase [EC:1.1.1.311] | Rhizobiales bacterium |
 ko:K18092 etbD; 2-hydroxy-6-oxo-octa-2,4-dienoate hydrolase [EC:3.7.1.-] | Mesorhizobium albiziae |
Nitrotoluene degradation | |
 ko:K10680 nemA; N-ethylmaleimide reductase [EC:1.-.-.-] | Azospirillum sp. TSA6c; Azospirillum doebereinerae |