Table 4 Substrate specificity of the partially purified NYSO laccase
Substrates | λmax (nm) | Extinction coefficient ɛ (mM−1 cm−1) | Activitya (U/L) (mean ± SD) | |
|---|---|---|---|---|
1 mM | 20 mM | |||
ABTS | 436 | 29.3 | 1300 ± 9.02 | 7271 ± 6.98 |
Catechol | 450 | 26 | 922 ± 8.49 | 4378 ± 5.77 |
Pyrogallol | 450 | 35 | 785 ± 4.11 | 3336 ± 4.97 |
p-anisidine | 350 | 1.173 | 26546 ± 108.7 | 83847 ± 50.57 |
4-nitrophenol | 405 | 18.1 | 4270 ± 8.16 | 7920 ± 8.16 |
Hydroquinone | 525 | 17.542 | 1318 ± 4.05 | 2585 ± 8.61 |
4-aminophenol | 470 | 3.4 | 7074 ± 8.99 | 26390 ± 69.76 |
p.phylenediamine | 525 | 14.68 | 11512 ± 15.14 | 19250 ± 122.5 |
Syringaldazine | 530 | 65 | 744 ± 11.43 | 2179 ± 3.09 |
Guaiacol | 465 | 26.6 | 801 ± 7.25 | 2446 ± 11.05 |
Tannic acid | 458 | 27.200 | 1415 ± 14.72 | 3340 ± 4.9 |
Vanillic acid | 390 | 2.340 | 14606 ± 76.54 | 26786 ± 84.01 |