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Fig. 10 | Journal of Genetic Engineering and Biotechnology

Fig. 10

From: Excavating the functionally crucial active-site residues of the DXS protein of Bacillus subtilis by exploring its closest homologues

Fig. 10

Schematic representation of the active site residues of the representative structure 6OUW. a Active site protein pocket yielded by CastP [45]. b Conservation profile of Consurf [46], maroon being the most conserved residue. c MI network [47] of the conserved coevolved residues encoded in 63 DXS sequences. a Labels outward of the second circle represent the residue loci, and the colored square boxes indicate the level of sequence conservation within the intensity range of low (blue) to high (red). The two internal circles show proximity mutual information and cumulative mutual information scores respectively. As per MISTIC protocol, the curved central linkers connect residues with statistically significant MI scores (> 6.5), with red, black, and gray orderly indicating the residue pairs with the top 5% scores, average scores between 70 and 95% and lowest scores. d Structural localization of the 10 conserved coevolving residues, indicating the most preserved for directed evolution methodologies

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