Table 9 In silico PCR analysis of primer pair catAf/catAr with targeted sequences
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Bacterial strains | Accession numbers | Product size | Product length | Primer dimer Tm (°C) |
|---|---|---|---|---|
Pseudomonas aeruginosa DSM 50071 | NZ_CP012001.1 | 365 | 308-672 | 24.0 |
Cupriavidus necator N-1 | NC_015726.1 | 371 | 299-669 | 24.0 |
Burkholderia ubonensis MSMB22 | NZ_CP009486.1 | 371 | 296-666 | 24.0 |
Burkholderia anthina strain AZ-4-2-10-S1-D7 | NZ_CM003769.1 | 374 | 323-696 | 24.0 |
Pseudomonas azotoformans S4 | NZ_CP014546.1 | 371 | 308-672 | 24.0 |
Pseudomonas lactis strain DSM 29167 | NZ_JYLO01000007.1 | 365 | 379-743 | 24.0 |
Pseudomonas simiae PCL1751 | NZ_CP010896.1 | 365 | 308-672 | 24.0 |
Acinetobacter johnsonii XBB1 | NZ_CP010351.1 | 371 | 296-666 | 24.0 |
Acinetobacter pittii PHEA-2 | NC_016603.1 | 371 | 296-666 | 24.0 |
Rhodococcus qingshengii djl-6-2 | NZ_CP025959.1 | 371 | 299-666 | 24.0 |
Rhodococcus opacus B4 | NC_012522.1 | 371 | 299-669 | 24.0 |
Pseudomonas brassicacearum subsp. brassicacearum | NC_015379.1 | 365 | 308-672 | 24.0 |
NFM421 Burkholderia thailandensis E264 | NC_007650.1 | 365 | 287-651 | 24.0 |
Burkholderia cenocepacia J2315 | NC_011002.1 | 374 | 314-687 | 24.0 |