Table 8 In silico PCR analysis of primer pair catAf/catAr with targeted sequences
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Bacterial strains | Accession numbers | Product size | Product length | Primer dimer Tm (°C) |
|---|---|---|---|---|
Ensifer adhaerens Casida A | NZ_CP015881.1 | 374 | 308-681 | 24.0 |
Pluralibacter gergoviae FB2 | NZ_CP009450.1 | 371 | 296-666 | 24.0 |
Burkholderia gladioli ATCC 10248 | NZ_CP009322.1 | 365 | 293-657 | 24.0 |
Klebsiella quasipneumoniae strain ATCC 700603 isolate K6 | NZ_CP014696.2 | 365 | 308-672 | 24.0 |
Pseudomonas putida NBRC 14164 | NC_021505.1 | 365 | 308-672 | 24.0 |
Acinetobacter venetianus VE-C3 | NZ_CM001772.1 | 371 | 296-666 | 24.0 |
Pseudomonas monteilii B5 | NZ_CP022562.1 | 365 | 308-672 | 24.0 |
Burkholderia lata | NC_007509.1 | 374 | 317-690 | 24.0 |
Acinetobacter indicus CIP 110367 | NZ_KI530745.1 | 371 | 296-666 | 24.0 |
Acinetobacter junii 65 | NZ_CP019041.1 | 371 | 296-666 | 24.0 |
Acinetobacter johnsonii XBB1 | NZ_CP010350.1 | 371 | 296-666 | 24.0 |
Pseudomonas protegens CHA0 | NC_021237.1 | 365 | 308-672 | 24.0 |
Cupriavidus metallidurans CH34 | NC_007974.2 | 371 | 299-669 | 24.0 |
Acinetobacter radioresistens DSM 6976 | NZ_KB849749.1 | 371 | 296-666 | 24.0 |
Pseudomonas aeruginosa PAO1 | NC_002516.2 | 365 | 308-672 | 24.0 |
Sphingomonas sp. KA1 | NC_008308.1 | 371 | 302-672 | 24.0 |