Table 6 In silico PCR analysis of primer pair catAf/catAr with targeted sequences
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Bacterial strains | Accession numbers | Product size | Product length | Primer dimer Tm (°C) |
|---|---|---|---|---|
Pseudomonas sp. ND6 | NC_005244.2 | 371 | 308–678 | 24.0 |
Acinetobacter baylyi ADP1 | NC_005966.1 | 374 | 373–746 | 24.0 |
Cupriavidus necator N-1 | NC_015727.1 | 366 | 286–651 | 24.0 |
Pseudomonas poae RE*1-1-14 | NC_020209.1 | 365 | 308–672 | 24.0 |
Acinetobacter colistiniresistens NIPH 2036 | NZ_KE340381.1 | 371 | 376–746 | 24.0 |
Burkholderia lata | NC_007511.1 | 365 | 385–749 | 24.0 |
Pseudomonas fulva 12-X | NC_015556.1 | 365 | 379–743 | 24.0 |
Pseudomonas stutzeri | NC_015740.1 | 371 | 370–740 | 24.0 |
Ralstonia mannitolilytica SN82F48 | NZ_CP010799.1 | 374 | 308–681 | 24.0 |
Burkholderia pseudomultivorans SUB-INT23-BP2 | NZ_CP013377.1 | 374 | 373–746 | 24.0 |
Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603 | NZ_CP027746.1 | 365 | 308–672 | 24.0 |
Burkholderia stagnalis MSMB735WGS | NZ_CP013461.1 | 365 | 287–651 | 24.0 |
Burkholderia territorii strain RF8-non-BP5 | NZ_CP013365.1 | 365 | 287–651 | 24.0 |
Burkholderia anthina strain AZ-4-2-10-S1-D7 | NZ_LNJP01000003.1 | 365 | 385–651 | 24.0 |
Burkholderia contaminans MS14 | NZ_CP009745.1 | 374 | 317–690 | 24.0 |
Pseudomonas savastanoi pv. savastanoi NCPPB 3335 | NZ_CP008742.1 | 365 | 403-767 | 24.0 |