Table 5 Degenerate primer evaluation for secondary structure
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Primers | Predicted secondary structure | Tm (°C) | ∆G kcal/mol |
|---|---|---|---|
catAf | – | – | – |
catAr | Internal or 5'end dimer | 36.8 | −15.0 |