Table 4 Degenerate PCR primer properties
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Primers | M.W (g/mol) | ∆H (kcal/mol) | ∆S (cal/kmol) | Tm (°C) | Tm(ATP) (°C) | ε L/(mol.cm) | nmol/OD260 |
|---|---|---|---|---|---|---|---|
catAf | 7018 | −175.4 | −496.8 | 58.0 | 60.6 | 221233 | 4.520 |
catAr | 7177 | −180.7 | −506.9 | 61.6 | 62.4 | 231625 | 4.317 |