Table 3 Degenerate PCR primer properties
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
Primers | %GC | A | G | T | C | μg/OD260 | L.C (%) | PPe (%) | ∆G kcal/mol |
|---|---|---|---|---|---|---|---|---|---|
catAf | 52.9 | 5.8 | 5.3 | 5.0 | 6.8 | 31.722 | 88 | 87 | −29.0 |
catAr | 57.2 | 6.3 | 9.6 | 3.6 | 3.6 | 30.985 | 95 | 51 | −31.1 |