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Table 12 In silico PCR result of primer pair catAf/catAr with authentic bacterial strains with (positive control) catechol 1,2-dioxygenase (C12O) gene

From: In silico design and validation of a highly degenerate primer pair: a systematic approach

No.

Bacterial strains

Accession numbers

Amplification results

catA

1

Burkholderia pyrrocinia DSM 10685

NZ_CP011504.1

+

2

Raoultella terrigena NCTC13098

NZ_LR131271.1

+

3

Pseudomonas yamanorum LBUM636

NZ_CP012400.2

+

4

Xanthomonas arboricola 17

NZ_CP011256.1

+

5

Pseudomonas otitidis DSM 17224

NZ_FOJP01000011.1

+

6

Pseudomonas frederiksbergensis strain ERDD5

NZ_CP017886.1

+

7

Klebsiella quasivariicola KPN1705

NZ_CP022823.1

+

8

Cupriavidus taiwanensis LMG 19424

NC_010530.1

±

9

Achromobacter insolitus DSM 23807

NZ_CP019325.1

+

10

Rhodococcus fascians D188

NZ_CP015235.1

+

  1. + Amplification, ± Partially amplified