Fig. 10
From: In silico design and validation of a highly degenerate primer pair: a systematic approach
In silico PCR result showing amplification of catechol 1,2-dioxygenases (C12O) gene with HYDEN designed degenerate primer catAf and catAr. The thick dark parallel non-overlapping lines in the figure represent both strands (Sense and antisense) of DNA in the orientation 5’-3’ and 3’-5’ respectively. The right-angle triangles depict the primers. Green right-angle triangles indicate primer-template binding in the 5’region of the sense strand while inverted olive green right-angle triangle illustrates primer-template binding in the 5’region of the antisense strand. All yellow figures depict mismatches between sequences in the primers and target