Fig. 5

The schematic diagram of PCR indicating the position of the adaptor primer and phosphate group followed by ligation reaction; the double-strand blue adaptor and genomic DNA digested with Bgl II enzyme. 5′ phosphate group sticky end creates in adaptor and DNA. After ligation, the first GSP1 primer (yellow arrow) binds to genomic DNA, extends ssDNA, and provides a site to anneal AP1 (black arrow). The short adaptor fragment does not have the primer site, and it cannot be as a template. In the next steps, PCR extends the desire product