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Journal of Genetic Engineering and Biotechnology

Table 4 IC50 values and phenol content for the T. indica fractions ability to inhibit the activity of purified GSTs

From: Antioxidant or pro-oxidant and glutathione transferase P1-1 inhibiting activities for Tamarindus indica seeds and their cytotoxic effect on MCF-7 cancer cell line

Extract of T. indica

IC50 value

Erythrocyte GST

Placenta GST

hGSTP1-1

Dichloromethane

Concentration (μg/ml)

64.54 ± 5.85

105.4 ± 3.3

93.74 ± 4.3

Phenol (μg GAE/g DW)

3.15 ± 0.28 (4.9%)

5.14 ± 0.16 (4.9%)

4.6 ± 0.2 (4.9%)

Flavonoid (mg RE/g DW)

2 ± 0.18 (3.1%)

3.2 ± 0.1 (3%)

2.9 ± 0.006 (3.1%)

Ethyl acetate

Concentration (μg/ml)

27 ± 1

56.66 ± 16.5

58 ± 15

Phenol (μg GAE/g DW)

3.3 ± 0.12 (12.2%)

6.9 ± 2 (12.2%)

7.08 ± 1.8 (12.2)

Flavonoid (mg RE/g DW)

1.3 ± 0.023 (4.8%)

1.3 ± 0.37 (2.3%)

1.33 ± 0.35 (2.3%)

n-butanol

Concentration (μg/ ml)

3 ± 0.7

6.6 ± 1.2

4.85 ± 0.35

Phenol (μg GAE/g DW)

1.13 ± 0.26 (37.6%)

2.5 ± 0.45 (37.9%)

1.83 ± 0.13 (37.7%)

Flavonoid (mg RE/g DW)

0.25 ± 0.05 (8.3%)

0.55 ± 0.09 (8.3%)

0.4 ± 0.03 (8.2)

  1. IC50: amount of extract which causes 50% inhibition of enzyme activity
  2. μg GAE/g DW: μg of gallic acid equivalent per gram of extract dry weight
  3. μg RE/g DW: μg of rutin equivalent per gram of extract dry weight
  4. The values represent the mean ± SD of three independent experiments
  5. The values in parentheses represent the percentage relative to concentration in μg/ml
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