Fig. 5

SDS-PAGE and immunoblot analysis of the polyclonal anti-RHT-D1A antibody by means of native and denatured rRHT-D1A proteins. a Five hundred nanograms of each protein was subjected to SDS-PAGE in a 10% gel and visualized by Coomassie brilliant blue staining. M: standard protein molecular weight markers. b Dot blot analysis of the anti-6xHis-RHT-D1A antibody against the native 6xHis-RHT-D1A recombinant protein. The anti-RHT-D1A, anti-APE1L, and anti-His antibodies were evaluated by the dot blot assay of purified 6xHis-RHT-D1A, a lysate of E. coli cells expressing 6xHis-RHT-D1A, and purified 6xHis-APE1L, which were spotted on PVDF membranes. c Western blot analysis of the polyclonal anti-RHT-D1A antibody against denatured rRHT-D1A proteins. The anti-RHT-D1A, anti-APE1L, and anti-His antibodies were evaluated by western blot analysis of 6xHis-RHT-D1A and 6xHis-APE1L. In all three western blots, 1 μg of purified 6xHis-RHT-D1A was loaded in the first lane and 0.5 μg of purified 6xHis-APE1L in the second lane